Protocol for high-sensitivity/long linear-range spectrofluorimetric DNA quantification using ethidium bromide
نویسندگان
چکیده
منابع مشابه
Protocol for high-sensitivity/long linear-range spectrofluorimetric DNA quantification using ethidium bromide.
Ethidium bromide (EtBr) is the most widely used fluorescent dye in nucleic acid gel electrophoresis since decades. However it has been essentially forgotten in DNA quantification by spectrofluorimetry. While investigating sensitivity and dynamic range of available fluorochromes, we found that EtBr permits much more sensitive fluorimetric measurements than previously thought. We report here a re...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2007
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000112500